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Ph stat download
Ph stat download






ph stat download

Linko YY, Javanainen P (1996) Simultaneous liquefaction, saccharification, and lactic acid fermentation on barley starch. Lee SY, Park JH, Jang SH, Nielsen LK, Kim J, Jung KS (2008) Fermentative butanol production by clostridia. Lee TM, Ishizaki A, Yoshino S, Furukawa K (1995) Production of acetone, butanol and ethanol from palm oil waste by Clostridium saccharoperbutylacetonicum N1-4. Jones DT, Woods DR (1986) Acetone-butanol fermentation revisited. Ishizaki A, Michiwaki S, Crabbe E, Kobayashi G, Sonomoto K, Yoshino S (1999) Extractive acetone-butanol-ethanol fermentation using methylated crude palm oil as extractant in batch culture of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Hüsemann MH, Papoutsakis ET (1990) Effects of propionate and acetate additions on solvent production in batch cultures of Clostridium acetobutylicum. Hüsemann MHW, Papoutsakis ET (1989) Enzymes limiting butanol and acetone formation in continuous and batch cultures of Clostridium acetobutylicum. Hofvendahl K, Hahn-Hägerdal B (1997) l-lactic acid production from whole wheat flour hydrolysate using strains of Lactobacilli and Lactococci. Hartmanis MGN, Klason T, Gatenbeck S (1984) Uptake and activation of acetate and butyrate in Clostridium acetobutylicum.

ph stat download

Hartmanis MGN, Gatenbeck S (1984) Intermediary metabolism in Clostridium acetobutylicum: levels of enzymes involved in the formation of acetate and butyrate. Harris LM, Desai RP, Welker NE, Papoutsakis ET (2000) Characterization of recombinant strains of the Clostridium acetobutylicum butyrate kinase inactivation mutant: need for new phenomenological models for solventogenesis and butanol inhibition? Biotechnol Bioeng 67:1–11 Green EM, Boynton ZL, Harris LM, Rudolph FB, Papoutsakis ET, Bennett GN (1996) Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824. J Biotechnol 115:179–187įond O, Matta-Ammouri G, Petitdemange H, Engasser JM (1985) The role of acids on the production of acetone and butanol by Clostridium acetobutylicum. Biotechnol J 2:1525–1534Įzeji TC, Qureshi N, Blaschek HP (2005) Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101. Metab Eng 1:206–213ĭürre P (2007) Biobutanol an attractive biofuel. J Ind Microbiol Biotechnol 36:319–332ĭesai RP, Harris LM, Welker NE, Papoutsakis ET (1999) Metabolic flux analysis elucidates the importance of the acid-formation pathways in regulating solvent production by Clostridium acetobutylicum. Appl Environ Microbiol 65:499–505ĭemain AL (2009) Biosolutions to the energy problem. Eur J Appl Microbiol Biotechnol 14:17–20Ĭhen CK, Blaschek HP (1999) Effect of acetate on molecular and physiological aspects of Clostridium beijerinckii NCIMB 8052 solvent production and strain degeneration. Eur J Appl Microbiol Biotechnol 18:327–332īahl H, Andersch W, Braun K, Gottschalk G (1982) Effect of pH and butyrate concentration on the production of acetone and butanol by Clostridium acetobutylicum grown in continuous culture. The results of the GC-MS analysis confirmed the bioconversion of lactic acid to butanol.Īndersch W, Bahl H, Gottschalk G (1983) Level of enzymes involved in acetate, butyrate, acetone and butanol formation by Clostridium acetobutylicum. To confirm whether lactic acid was converted to butanol by the N1-4 strain, we performed gas chromatography–mass spectroscopy (GC-MS) analysis of butanol produced by a batch culture during fermentation in a medium containing lactic acid as the initial substrate. Finally, we obtained 15.5 g/l butanol at a production rate of 1.76 g/l/h using a fed-batch culture with a pH-stat continuous lactic acid and glucose feeding method. In addition, a pH-controlled fed-batch culture resulted in not only acceleration of lactic acid consumption but also a further increase in butanol production. It was found that when fed a mixture of lactic acid and glucose, the final concentration of butanol produced by a fed-batch culture was greater than that produced by a batch culture. When a medium containing 20 g/l glucose was supplemented with 5 g/l of closely racemic lactic acid, both the concentration and yield of butanol increased however, supplementation with more than 10 g/l lactic acid did not increase the butanol concentration. In order to achieve high butanol production by Clostridium saccharoperbutylacetonicum N1-4, the effect of lactic acid on acetone–butanol–ethanol fermentation and several fed-batch cultures in which lactic acid is fed have been investigated.








Ph stat download